Morphological and biochemical alterations included muscle lipid accumulation, low muscle carnitine content, reduction in fatty acid β-oxidation and reduced activity of complexes I and II of the respiratory chain. We designed the comparison feature to analyze lanes and determine their similarity to generate even more robust data.In this case report we studied alterations in mitochondrial proteins in a patient suffering from recurrent profound muscle weakness, associated with ethylmalonic-adipic aciduria, who had benefited from high dose of riboflavin treatment. The comparison function analyzes and determines lane similarity using mathematical modeling. In-channel normalization: Calculates relative volume of bands to the volume of a reference band or user-selected reference lane.Ĭomparison feature: Generate robust data on lane similarity Housekeeping protein normalization: Identifies the largest band in the reference channel as the housekeeping band, then normalizes each lane against the housekeeping band as reference. Total protein normalization: Uses the total lane volume of all bands in the lane of a selected reference channel to normalize the bands in the same lane of other channels. Single band normalization: Normalizes all bands against a single chosen reference band. IQTL includes multiple normalization options to meet your needs for both single and multichannel images. Ideal for images generated by Amersham ImageQuant 800 and Amersham Typhoon imaging systems GxP version for regulated environments requiring data integrity and user group management Lane comparison to produce data like similarity score profiles and dendrograms Total protein normalization data with a single click Support for images in JPG, PNG, and TIF formatsĮditing functions and contrast adjustment options at every stage of analysis ImageQuant TL 10.0 analysis software: Overview
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